View source for Generating DMSO Stocks for Cell Culture

==Materials==
*Cells in 10cm dishes, at 90-95% confluence.
*Cryopreservation Container (Nalgene 5100-0001)
*Sterile DMSO

==Protocol==
#Pick a low passage number of cells and grow 2-5 10cm dishes.
#At near confluence wash cells twice with PBS -/- and trypsinize normally
#Collect all the cells in a 15 mL falcon tube
#Centrifuge 5 min at 1500RPM to pellet cells
#Aspirate media
#Add media (1.8 mL per original plate)
#Add sterile DMSO to a final concentration of 10% (0.2 mL per original plate)
#Gently resuspend cells and aliquot 1 mL of suspension into cryopreservation vials