View source for GoTaq PCR Genotyping

==Materials==
*GoTaq Master Mix
*Primer Mix (100uL Primers): Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20. 
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template 
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr. 
 

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==Procedure==
*Prepare gel 30 minutes before PCR is finished. 
*Prepare Reaction Mixture, adding 13x all materials except for the template. 
*Add this mixture to each PCR tube. 
*Label and add each template to the corresponding PCR tube. 
*Run PCR under genotyping>inoki (~1 hour) 
*Load samples in gel and run on 30V (horizontally.)