View source for Measuring Autophagy in Tissue Culture Cells

[[ Category: Tissue Culture ]]
[[ Category: Cell Culture ]]
[[ Category: Autophagy ]]
[[ Category: mTORC1 ]]

For detailed protocol see [http://dx.doi.org/10.4161/auto.19496 Klionsky et al, 2012]

To prevent turnover of autolysosomes, the terminal step of autolysosomal degradation can be blocked with Bafilomycin A1 treatment.  Typically this is done for 1-2h by adding it at 100 nM just prior to induction of autophagy.  This is a 1000X dilution of our 100 uM stock solution in DMSO.