View source for Sending Samples for DNA Sequencing

# Create an account or log in at http://128.169.4.12/
# Select Economy  under Service Requested and enter the number of samples in the box above.
# Select '''Enter Individual DNA Sequencing Requests'''
# Enter the tube and select the primers (you will have to provide the primers if doing economy).  Hit enter, print out the confirmation page for your notes.
# Write the template name on each of your tubes
# In each tube add:
* 1 uL of template (unless the plasmid DNA concentration is >500 ng/uL in which case dilute it to 50-300 ng/uL
* 3.2 uL of 1 uM Primer (Dilute the primer stock 100X)
* 7.8 uL of water

# Drop the samples off at the MRC.