View source for TC10b Genotyping

==Primers==
* '''TC10b - PDFor1''':  GGAGTCTATGGACATGAGAG
* '''TC10b - PD4-43''':  GATCATGACTAGCATGGAGTAG
* '''TC10b - PDHomoFB''':  GTCTATGGACATGAGAGTTTC
* '''TC10b - NEO3A''': GCAGCGCATCGCCTTCTATC

==Genotyping Program==
* 94 2min
* 50 cycles of:
** 94 30s
** 56 30s
** 72 4min
* 72 7min
* 4 forever

==Results==

WT allele ~ 1kb, KO allele ~ 500bp
HET should get two bands

[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]