View source for TSC-floxed Genotyping

==Primers==
* Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
* Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG

* TSC1 (F) AGG AGG CCT CTT CTG CTA CC
* TSC1 (R) CAG CTC CGA CCA TGA AGT G

==Reference==
From Kwiatkowski ''et al.'', Hum. Mol. Genet. 11(5):525-34.  2002.  A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in Tsc1 null cells.  http://www.ncbi.nlm.nih.gov/pubmed/11875047:

<blockquote>
PCR genotyping was performed by simultaneous amplification of both wild-type Tsc1 and the deleted allele using the following three primers in a 35 cycle PCR reaction using Perkin Elmer AmpliTaq Gold: F4536, 5′-AGGAGGCCTCTTCTGCTACC-3′; R4830, 5′-CAGCTCCGACCATGAAGTG-3′; and R6548, 5′-TGGGTCCTGACCTATCTCCTA-3′ (Fig. 1D). Products were 295 bp (wild-type) and 368 bp (mutant), and were analyzed on agarose gels.
</blockquote>

[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]
[[ Category:mTORC1 ]]