Difference between revisions of "Cre PCR"
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==PCR Program== | ==PCR Program== | ||
| + | |||
| + | * Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG | ||
| + | * Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG | ||
*95C 1min | *95C 1min | ||
| Line 8: | Line 11: | ||
*72C 5min | *72C 5min | ||
*4C forever | *4C forever | ||
| + | |||
| + | [[ Category:Genotyping ]] | ||
| + | [[ Category:Mouse Work]] | ||
| + | [[ Category:PCR]] | ||
| + | [[ Category:DNA]] | ||
| + | [[ Category:Molecular Biology]] | ||
Latest revision as of 18:42, 25 April 2018
PCR Program
- Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
- Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
- 95C 1min
- 35 Cycles of:
- 95C 10s
- 55C 30s
- 72C 45s
- 72C 5min
- 4C forever
