Difference between revisions of "Rapamycin Injections"
From Bridges Lab Protocols
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==Preparation== | ==Preparation== | ||
| − | *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip | + | *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. |
| − | *Dissolve rapamycin at 20 mg/mL | + | *Dissolve rapamycin at 20 mg/mL in water. |
| + | *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | ||
Revision as of 15:10, 28 July 2009
Materials
- Tween 80 - Sigma P1754
- PEG - Sigma P3265
Preparation
- Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.
- Dissolve rapamycin at 20 mg/mL in water.
- Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
