Difference between revisions of "User:Sotu"

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(Created page with "== Cholesterol Assay == === Materials === * Reagent * Cholesterol Standard === Protocol === * Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL...")
 
(Cholesterol Assay)
 
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== Cholesterol Assay ==
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=== Materials ===
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* Reagent
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* Cholesterol Standard
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=== Protocol ===
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* Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
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* Pipette 100ɥL of Reagent to 22 wells
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* Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
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* Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
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* Allow reaction to proceed for 15 minutes
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* Read absorbance
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Latest revision as of 13:50, 28 October 2023