Difference between revisions of "Finding and Ordering BAC Clones"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (initial page) |
Davebridges (Talk | contribs) m |
||
(5 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
==Find Clone with UCSD Genome Browswer== | ==Find Clone with UCSD Genome Browswer== | ||
#Go to [[http://genome.ucsc.edu UCSC Genome Browser]] | #Go to [[http://genome.ucsc.edu UCSC Genome Browser]] | ||
− | #Click on Genome | + | #Click on Genome Browser |
+ | #Select Mouse (or whatever species you need) under genome | ||
+ | #Type gene name in the search box | ||
+ | #Select appropriate item from search results | ||
+ | #Adjust tracks as necessary, being sure to include BAC End Pairs under Mapping and Sequence Tracks | ||
+ | #Zoom out as necessary to find ends of BAC clones | ||
+ | #Print out and save BAC clone choices | ||
+ | |||
+ | ==Ordering BAC Clones== | ||
+ | #Go to BACPAC website (http://bacpac.chori.org/order_clones.php) | ||
+ | #Enter clone name and submit to verify | ||
+ | #Can order clones through LSI purchasing (vendor is listed as BACPAC-chor) | ||
+ | |||
+ | [[Category:Ordering]] | ||
+ | [[Category:Bioinformatics]] |
Latest revision as of 15:50, 28 July 2009
Find Clone with UCSD Genome Browswer
- Go to [UCSC Genome Browser]
- Click on Genome Browser
- Select Mouse (or whatever species you need) under genome
- Type gene name in the search box
- Select appropriate item from search results
- Adjust tracks as necessary, being sure to include BAC End Pairs under Mapping and Sequence Tracks
- Zoom out as necessary to find ends of BAC clones
- Print out and save BAC clone choices
Ordering BAC Clones
- Go to BACPAC website (http://bacpac.chori.org/order_clones.php)
- Enter clone name and submit to verify
- Can order clones through LSI purchasing (vendor is listed as BACPAC-chor)