Difference between revisions of "Dilution Formula"
From Bridges Lab Protocols
(Created page with 'C1V1=C2V2 C1=measured absorption V1=unknown volume to be added to final volume V2=desired volume to re-suspend V1 in (usually 4mL) C2=<math>OD/2^x</math> x=amount of times ce...') |
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C1V1=C2V2 | C1V1=C2V2 | ||
| − | C1=measured absorption | + | *C1=measured absorption |
| − | V1=unknown volume to be added to final volume | + | *V1=unknown volume to be added to final volume |
| − | V2=desired volume to re-suspend V1 in (usually 4mL) | + | *V2=desired volume to re-suspend V1 in (usually 4mL) |
| − | C2= | + | *C2=OD/2^x |
| − | x=amount of times cells will double | + | |
| − | + | *x=amount of times cells will double | |
| + | *find this by dividing the number of hours the cells will be growing by how long one doubling period is | ||
Latest revision as of 13:46, 29 June 2010
C1V1=C2V2
- C1=measured absorption
- V1=unknown volume to be added to final volume
- V2=desired volume to re-suspend V1 in (usually 4mL)
- C2=OD/2^x
- x=amount of times cells will double
- find this by dividing the number of hours the cells will be growing by how long one doubling period is
