Difference between revisions of "Rapamycin Injections"
From Bridges Lab Protocols
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Davebridges (Talk | contribs) (added dosage volume) |
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*Dissolve rapamycin at 20 mg/mL in water. | *Dissolve rapamycin at 20 mg/mL in water. | ||
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | ||
| + | *Inject 10 uL/g body weight | ||
Revision as of 18:02, 23 June 2011
Materials
- Tween 80 - Sigma P1754
- PEG - Sigma P3265
Preparation
- Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.
- Dissolve rapamycin at 20 mg/mL in water.
- Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
- Inject 10 uL/g body weight
