Difference between revisions of "Rapamycin Injections"

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(added dosage volume)
(added rapamycin treatment from Lamming et al)
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*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
 
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
 
*Inject 10 uL/g body weight
 
*Inject 10 uL/g body weight
 +
 +
==Alternate Rapamycin Injection Regimen==
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* Phenotypes seem to be different with rapamycin as treated by Lamming et al.
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* This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135
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<blockquote>
 +
rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally
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once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a
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concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
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</blockquote>

Revision as of 17:35, 3 September 2012

Materials

  • Tween 80 - Sigma P1754
  • PEG - Sigma P3265

Preparation

  • Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.
  • Dissolve rapamycin at 20 mg/mL in water.
  • Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
  • Inject 10 uL/g body weight

Alternate Rapamycin Injection Regimen

rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.