Difference between revisions of "Genotyping Program"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added ob/ob genotyping) |
Davebridges (Talk | contribs) (added note about band size in cap ko) |
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|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]] | |[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]] | ||
|- | |- | ||
| − | |[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote | + | |[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO |
|- | |- | ||
|[[Vinexin Genotyping|Vinexin]] || Vinexin || || | |[[Vinexin Genotyping|Vinexin]] || Vinexin || || | ||
Revision as of 15:22, 23 October 2012
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Inoki PCR | Two bands if heterozygote | |
| Cre | Inoki PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Inoki PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping |
| CAP | CAP | Two bands if heterozygote, upper band for KO | |
| Vinexin | Vinexin |
