Difference between revisions of "Rapamycin Injections"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added rapamycin treatment from Lamming et al) |
Davebridges (Talk | contribs) (updated vehicle information) |
||
| Line 1: | Line 1: | ||
==Materials== | ==Materials== | ||
*Tween 80 - Sigma P1754 | *Tween 80 - Sigma P1754 | ||
| − | *PEG - Sigma P3265 | + | *PEG 400 Liquid - Sigma P3265 |
==Preparation== | ==Preparation== | ||
| − | *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. | + | *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween |
*Dissolve rapamycin at 20 mg/mL in water. | *Dissolve rapamycin at 20 mg/mL in water. | ||
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | ||
Revision as of 16:31, 5 November 2012
Materials
- Tween 80 - Sigma P1754
- PEG 400 Liquid - Sigma P3265
Preparation
- Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
- Dissolve rapamycin at 20 mg/mL in water.
- Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
- Inject 10 uL/g body weight
Alternate Rapamycin Injection Regimen
- Phenotypes seem to be different with rapamycin as treated by Lamming et al.
- This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135
rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
