Difference between revisions of "MTM1 Genotyping"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (wrote protocol for MTM1 genotyping) |
Davebridges (Talk | contribs) (added sample pcr result) |
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==Expected Results== | ==Expected Results== | ||
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| + | [[File : MTM1_Genotyping_Result.jpg]] | ||
Revision as of 15:16, 21 January 2013
Primers
- MTM1 Fwd: GGC CCC ATT AAG GGA AGA GT
- MTM1 WT Rev: GAT CTT CTG GGA AAG GCA CA
- MTM1 Mut Rev: GCC AGA GGC CAC TTG TGT AG
PCR Program
- 94 for 2 min
- 10 cycles of:
- 94 for 20sec
- 65 for 15sec with -0.5 C per cycle
- 68 for 10sec
- 28 cycles of:
- 94 for 15sec
- 60 for 15sec
- 72 for 10sec
- 72 for 1 min
- 4 hold
