Difference between revisions of "Rapamycin Injections"
From Bridges Lab Protocols
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rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally | rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally | ||
| − | once daily with either | + | once daily with either 1 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a |
concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days. | concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days. | ||
</blockquote> | </blockquote> | ||
Revision as of 17:24, 17 February 2014
Materials
- Tween 80 - Sigma P1754
- PEG 400 Liquid - Sigma P3265
Preparation
- Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
- Dissolve rapamycin at 20 mg/mL in water.
- Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
- Inject 10 uL/g body weight
Alternate Rapamycin Injection Regimen
- Phenotypes seem to be different with rapamycin as treated by Lamming et al.
- This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135
rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally once daily with either 1 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
