Difference between revisions of "Dexamethasone Treatment of Mice"

From Bridges Lab Protocols
Jump to: navigation, search
m (Calculation)
m (Calculation)
 
(One intermediate revision by the same user not shown)
Line 32: Line 32:
 
* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
 
* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
 
<pre>  
 
<pre>  
65mg dex/                        =    6mg dex/                =>    X=  0.092g  
+
65mg dex/                        =    6mg dex/                =>    X=  0.092g solid powder
1000mg solid powder                  ? (X) mg powder   
+
1000mg solid powder                  ? (X) mg solid powder   
 
</pre>
 
</pre>
 
*This assumes that the mice are about 30g and they drink 5mL/day.
 
*This assumes that the mice are about 30g and they drink 5mL/day.

Latest revision as of 20:26, 30 December 2019


We use water-soluble Dex from Sigma (D2915). It says on the bottle how many mg/g is actually Dex. The calculations below are for a bottle where the powder was 65mg/g. Dex


For i.p. injection 5 mg/kg

  • Makes 15mL stock solution of 1mg/mL
  • dissolve 0.231g Dex powder in 15mL PBS
BW (g) X 0.005 = injection volume (mL)

Calculation

65mg dex/        =       15mg dex/        =  0.231g
1000mg powder          ? mg powder   

For drinking water administration ~1mg/kg/day

  • Makes 1L of drinking water
  • dissolve 0.092g in 1L water

Calculation

  • 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
  • average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
 
65mg dex/                         =    6mg dex/                =>    X=  0.092g solid powder
1000mg solid powder                   ? (X) mg solid powder   
  • This assumes that the mice are about 30g and they drink 5mL/day.