Difference between revisions of "Acrylamide Solution"

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[[Category:SDS-PAGE]]
 
[[Category:SDS-PAGE]]
 
[[Category:Electrophoresis]]
 
[[Category:Electrophoresis]]
[[Category:Buffers]]
+
[[Category:Buffer]]
 
For 1L:
 
For 1L:
*181.66g Tris
+
*290g Acrylamide
*40 mL 10% SDS (or 4 g SDS)
+
*10g Bis-Acrylamide
*Add water to near 1L
+
*Adjust pH to 8.8
+
 
*Bring volume up to 1L
 
*Bring volume up to 1L

Latest revision as of 18:57, 3 August 2009

For 1L:

  • 290g Acrylamide
  • 10g Bis-Acrylamide
  • Bring volume up to 1L