Difference between revisions of "Induction Conditions"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added hrs and fyve) |
Davebridges (Talk | contribs) (added Rab5) |
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| GST-HRS (2xFYVE) | | GST-HRS (2xFYVE) | ||
| + | | 10 uM | ||
| + | | Overnight | ||
| + | | 28C | ||
| + | | very soluble and easy to induce. | ||
| + | |- | ||
| + | | GST-Rab5 | ||
| 10 uM | | 10 uM | ||
| Overnight | | Overnight | ||
| Line 25: | Line 31: | ||
| very soluble and easy to induce. | | very soluble and easy to induce. | ||
|} | |} | ||
| + | [[Category:Protein Purification]] | ||
Latest revision as of 17:54, 24 July 2012
| Protein | IPTG | Time | Temperature | Notes |
|---|---|---|---|---|
| GST-PLC | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS (2xFYVE) | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-Rab5 | 10 uM | Overnight | 28C | very soluble and easy to induce. |
