Difference between revisions of "RIPA Buffer"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (changed vanadate concentration and volume) |
Davebridges (Talk | contribs) (→RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol) |
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! || Final Concentration || per 10 mL || Stock || Location | ! || Final Concentration || per 10 mL || Stock || Location | ||
|- | |- | ||
| − | |Tris pH7.4 || 50mM || 500uL || 1M || Shelf | + | |Tris pH7.4 || 50mM || 500uL || 1M || Chemical Shelf |
|- | |- | ||
| − | |Na Deoxycholate || 0.25% || 250uL || 10% || Shelf | + | |Na Deoxycholate || 0.25% || 250uL || 10% || Chemical Shelf |
|- | |- | ||
| − | | NP-40 || 1% || 1mL || 10% || | + | | NP-40 || 1% || 1mL || 10% || Chemical Shelf |
|- | |- | ||
| − | |NaCl || 150mM || 375uL || 4M || Shelf | + | |NaCl || 150mM || 375uL || 4M || Chemical Shelf |
|- | |- | ||
| − | |EDTA || 1mM || 20uL || 0.5M || Shelf | + | |EDTA || 1mM || 20uL || 0.5M ||Chemical Shelf |
|- | |- | ||
| − | |NaVO3 || 100uM || 10uL || 100mM || | + | |NaVO3 (see [[Preparation_of_Orthovanadate_Stocks | preparation ]]) || 100uM || 10uL || 100mM || Chemical Shelf |
|- | |- | ||
| − | |NaF || 5mM || 100uL || 500mM || Shelf | + | |NaF || 5mM || 100uL || 500mM || Chemical Shelf |
|- | |- | ||
| − | |NaPPi || | + | |NaPPi || 10 mM || 1 mL || 100mM || Chemical Shelf |
|- | |- | ||
| Protease Inhibitors || || 1 mini tablet || || 4C | | Protease Inhibitors || || 1 mini tablet || || 4C | ||
|} | |} | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||
Latest revision as of 20:57, 27 January 2016
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M | Chemical Shelf |
| Na Deoxycholate | 0.25% | 250uL | 10% | Chemical Shelf |
| NP-40 | 1% | 1mL | 10% | Chemical Shelf |
| NaCl | 150mM | 375uL | 4M | Chemical Shelf |
| EDTA | 1mM | 20uL | 0.5M | Chemical Shelf |
| NaVO3 (see preparation ) | 100uM | 10uL | 100mM | Chemical Shelf |
| NaF | 5mM | 100uL | 500mM | Chemical Shelf |
| NaPPi | 10 mM | 1 mL | 100mM | Chemical Shelf |
| Protease Inhibitors | 1 mini tablet | 4C |
