Difference between revisions of "Induction Conditions"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Created page with '{| class="wikitable" |- ! Protein ! IPTG Concentration ! Time ! Temperature ! Notes |- | some protein | some IPTG | some time | some temperature | some note |- | row 2, cel...') |
Davebridges (Talk | contribs) (added Rab5) |
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| − | {| class="wikitable" | + | {| class="wikitable" border= "1" |
|- | |- | ||
! Protein | ! Protein | ||
| − | ! IPTG | + | ! IPTG |
! Time | ! Time | ||
! Temperature | ! Temperature | ||
! Notes | ! Notes | ||
|- | |- | ||
| − | | | + | | GST-PLC |
| − | | | + | | 10 uM |
| − | | | + | | Overnight |
| − | | | + | | 28C |
| − | | | + | | very soluble and easy to induce. |
|- | |- | ||
| − | | | + | | GST-HRS |
| − | | | + | | 10 uM |
| − | | | + | | Overnight |
| + | | 28C | ||
| + | | very soluble and easy to induce. | ||
| + | |- | ||
| + | | GST-HRS (2xFYVE) | ||
| + | | 10 uM | ||
| + | | Overnight | ||
| + | | 28C | ||
| + | | very soluble and easy to induce. | ||
| + | |- | ||
| + | | GST-Rab5 | ||
| + | | 10 uM | ||
| + | | Overnight | ||
| + | | 28C | ||
| + | | very soluble and easy to induce. | ||
|} | |} | ||
| + | [[Category:Protein Purification]] | ||
Latest revision as of 17:54, 24 July 2012
| Protein | IPTG | Time | Temperature | Notes |
|---|---|---|---|---|
| GST-PLC | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS (2xFYVE) | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-Rab5 | 10 uM | Overnight | 28C | very soluble and easy to induce. |
