Difference between revisions of "PCR Analysis of Tail DNA"
From Bridges Lab Protocols
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Run "specfic" PCR Program for gene of interest (approx 2 hours). | Run "specfic" PCR Program for gene of interest (approx 2 hours). | ||
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Revision as of 18:37, 17 February 2016
see Genotyping Details for strain specific details
Materials
- Dream Taq Green master mix
- Specific Primers
- DNA
- ddH2O
Protocol
Use the following Volumes per 25ul Reaction:
Per sample 1X
- Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
- Primer Mix: 5ul
- Sterile ddH2O: 7.5ul
- Template: 1 uL
Run "specfic" PCR Program for gene of interest (approx 2 hours).
see Preparing an Agarose Gel for details on preparing a DNA gel
