Difference between revisions of "Glucose Tolerance Test"
From Bridges Lab Protocols
Davebridges (Talk | contribs) |
Davebridges (Talk | contribs) (added glucose strip ordering information) |
||
| Line 1: | Line 1: | ||
==Materials (Bring Downstairs)== | ==Materials (Bring Downstairs)== | ||
*Glucometer - AccuChek Advantage | *Glucometer - AccuChek Advantage | ||
| − | *Glucose Test Strips - AccuChek Comfort Curve | + | *Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]] |
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
| Line 14: | Line 14: | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | ||
| − | + | ##Immobilize mouse and restrain tail with one hand | |
| − | + | ##Aim needle between the midline and the hip bone | |
| − | + | ##Insert syringe (do not inject) into cavity | |
| − | + | ##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | |
| − | + | ##Eject syringe. | |
#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | #At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | ||
Revision as of 13:41, 10 June 2009
Materials (Bring Downstairs)
- Glucometer - AccuChek Advantage
- Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
- Timer
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room. Put water bottle on cage.
- Weigh mice, mark tails as necessary and take fasting glucose measurement.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
- Immobilize mouse and restrain tail with one hand
- Aim needle between the midline and the hip bone
- Insert syringe (do not inject) into cavity
- Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
- At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein
