Difference between revisions of "RIPA Buffer"

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(Created page with '==RIPA Buffer (for 10mL lysis buffer)== {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- |Tris pH7.4 || 50mM || 500uL || 1M |- |Na Deoxycholate || 0....')
 
(changed vanadate concentration and volume)
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! || Final Concentration || per 10 mL || Stock || Location
 
! || Final Concentration || per 10 mL || Stock || Location
 
|-  
 
|-  
−
|Tris pH7.4 || 50mM || 500uL || 1M
+
|Tris pH7.4 || 50mM || 500uL || 1M || Shelf
 
|-  
 
|-  
−
|Na Deoxycholate || 0.25% || 250uL || 10%
+
|Na Deoxycholate || 0.25% || 250uL || 10% || Shelf
 
|-
 
|-
−
| NP-40 || 1% || 1mL || 10%
+
| NP-40 || 1% || 1mL || 10% || Tingting
 
|-  
 
|-  
−
|NaCl || 150mM || 375uL || 4M
+
|NaCl || 150mM || 375uL || 4M || Shelf
 
|-  
 
|-  
−
|EDTA || 1mM || 20uL || 0.5M
+
|EDTA || 1mM || 20uL || 0.5M || Shelf
 
|-  
 
|-  
−
|NaVO3 || 1mM || 100uL || 1M || Biochemical Stocks (-20)
+
|NaVO3 || 100uM || 10uL || 100mM || Biochemical Stocks (-20)
 
|-  
 
|-  
−
|NaF || 5mM || 100uL || 500mM
+
|NaF || 5mM || 100uL || 500mM || Shelf
 
|-
 
|-
−
|NaPPi || 25 mM || 1 mL || 250mM
+
|NaPPi || 25 mM || 1 mL || 250mM || Shelf
 
|-
 
|-
 
| Protease Inhibitors || || 1 mini tablet || || 4C
 
| Protease Inhibitors || || 1 mini tablet || || 4C
 
|}
 
|}
 
[[ Category:Buffer ]]
 
[[ Category:Buffer ]]

Revision as of 15:28, 21 December 2009

RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M Shelf
Na Deoxycholate 0.25% 250uL 10% Shelf
NP-40 1% 1mL 10% Tingting
NaCl 150mM 375uL 4M Shelf
EDTA 1mM 20uL 0.5M Shelf
NaVO3 100uM 10uL 100mM Biochemical Stocks (-20)
NaF 5mM 100uL 500mM Shelf
NaPPi 25 mM 1 mL 250mM Shelf
Protease Inhibitors 1 mini tablet 4C
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=RIPA_Buffer&oldid=442"