Difference between revisions of "Making LB Agar Plates"
From Bridges Lab Protocols
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| + | ==SOP== | ||
| + | *[[SOP- Autoclave]] | ||
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==Materials== | ==Materials== | ||
* Petri Dishes | * Petri Dishes | ||
Latest revision as of 15:28, 8 June 2020
SOP
Materials
- Petri Dishes
- Bacto Agar (BD 214010)
- LB Broth (Sigma L3522)
- Antibiotic, usually at 1000X, add 1 uL/mL of media
Protocol
- 500 mL makes about one sleeve of petri dishes
- Add 25g LB Broth per Liter to an appropriately sized beaker
- Add Water and Stir until clumps are gone
- Add 15 g Agar per Liter , this will not dissolve
- Transfer to a erlenmeyer flask at least 2x the volume that you are making
- Autoclave for at least a 20 minute liquid cycle
- Once the liquid is cool to the touch (dosent burn your hand to hold) add antibiotic and mix by swirling
- Pour into plates, covering the surface and avoiding bubbles
- Burn off bubbles with bunsen burner or pop with a sterile tip
- Let plates cool with the lids ajar
- Invert plates, place in sleeve and mark sleeve as LB/Amp or LB/Km depending on the antibiotic
