Difference between revisions of "TaqMan SNP Assay"
From Bridges Lab Protocols
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== Materials == | == Materials == | ||
| − | TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | + | *TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). |
| − | + | *TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems). | |
| − | TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems). | + | *MicroAmp Fast 96-Well Plates. |
| − | + | *Optical Plate Covers. | |
| − | MicroAmp Fast 96-Well Plates. | + | *StepOnePlus Real-Time PCR System (Applied Biosystems). |
| − | + | *PC/Laptop with StepOnePlus Software installed | |
| − | Optical Plate Covers. | + | |
| − | + | ||
| − | StepOnePlus Real-Time PCR System (Applied Biosystems). | + | |
| − | + | ||
| − | PC/Laptop with StepOnePlus Software installed | + | |
| − | + | ||
== Protocol == | == Protocol == | ||
Revision as of 15:57, 11 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed
