Difference between revisions of "Preparation of Orthovanadate Stocks"

Revision as of 20:56, 27 January 2016

Protocol from http://www.abcam.com/ps/pdf/protocols/Buffers%20and%20stock%20solutions.pdf

This needs to be done in the fume hood

1. Prepare a 100 mM solution in double distilled water (1.83g/100mL).
2. Set pH to 9.0 with HCl, color will become yellow
3. Boil until colorless.
4. Cool to room temperature.
5. Set pH to 9.0 again.
6. Boil again until colorless.
7. Repeat this cycle until the solution remains at pH 9.0 after boiling and cooling.
8. Bring up to the initial volume with water.
9. Store in aliquots at -20°C in the box marked Protease Inhibitors

Note: do not permit great changes in volume during boiling; put a loose lid on the container to protect from evaporation. Discard if the samples turn yellow.