Difference between revisions of "PCR Analysis of Tail DNA"
From Bridges Lab Protocols
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==Protocol== | ==Protocol== | ||
| − | Use the following Volumes per | + | Use the following Volumes per 25ul Reaction: |
| − | # | + | Per sample 1X |
| + | #Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer) | ||
#Primer Mix: 5ul | #Primer Mix: 5ul | ||
| − | + | #Sterile ddH2O: 7.5ul | |
| − | #Sterile | + | |
| − | + | ||
| − | + | ||
| − | + | *Template: 1 uL | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | * | + | |
| − | |||
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| − | + | Run "specfic" PCR Program for gene of interest (approx 2 hours). | |
| + | * specific to each gene | ||
| + | |||
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | ||
Revision as of 18:36, 17 February 2016
see Genotyping Details for strain specific details
Materials
- Dream Taq Green master mix
- Specific Primers
- DNA
- ddH2O
Protocol
Use the following Volumes per 25ul Reaction:
Per sample 1X
- Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
- Primer Mix: 5ul
- Sterile ddH2O: 7.5ul
- Template: 1 uL
Run "specfic" PCR Program for gene of interest (approx 2 hours).
- specific to each gene
see Preparing an Agarose Gel for details on preparing a DNA gel
