Difference between revisions of "50X TAE Buffer"
From Bridges Lab Protocols
| Line 5: | Line 5: | ||
*18.6g EDTA | *18.6g EDTA | ||
| − | Add Tris Base, Glacial Acetic Acid, and EDTA to | + | 1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. |
| + | 2. Add remaining 400mL water and mix on stir plate (or my swirling). | ||
[[Category: Buffers]] | [[Category: Buffers]] | ||
[[Category: Genotyping]] | [[Category: Genotyping]] | ||
Revision as of 15:20, 9 September 2016
Reagents
- 900mL ddH2O
- 242g Tris Base
- 57.1 mL Glacial Acetic Acid
- 18.6g EDTA
1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. 2. Add remaining 400mL water and mix on stir plate (or my swirling).
