Difference between revisions of "Glucose Tolerance Test"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (wrote initial page) |
Davebridges (Talk | contribs) (→Materials (Bring Downstairs)) |
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*Beaker for weighing mice | *Beaker for weighing mice | ||
*Syringes | *Syringes | ||
| − | *10% Glucose in PBS (sterile filtered). This will correspond to 1g/kg glucose injections. | + | *10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. |
==Protocol== | ==Protocol== | ||
Revision as of 15:41, 13 May 2009
Materials (Bring Downstairs)
- Glucometer
- Glucose Test Strips
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room.
- Weigh mice, mark tails as necessary and take fasting glucose measurement.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
- Immobilize mouse and restrain tail with one hand
- Aim needle between the midline and the hip bone
- Insert syringe (do not inject) into cavity
- Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
