Difference between revisions of "FDB Muscle Dissection from Mice"
From Bridges Lab Protocols
m (Snyderds moved page Fdb muscle dissection from mice to FDB Muscle Dissection from Mice) |
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| − | This protocol is modified from section 3 | + | This protocol is modified from section 3.1.2 of [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523695/ Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells] and the first part of the following video with accompanying protocol [https://www.jove.com/video/53551/dna-electroporation-isolation-and-imaging-of-myofibers DNA Electroporation, Isolation and Imaging of Myofibers | Protocol]. |
<big>'''Materials'''</big> | <big>'''Materials'''</big> | ||
| − | * | + | * Tweezers |
| + | * Standard operating scissors | ||
| + | * Surgical scissors | ||
| + | * Forceps | ||
| + | * Dissection stage with 7 pins | ||
| + | * Anesthetic | ||
| + | |||
| + | <big>'''Flexor Digitorum Brevis Muscle Extraction'''</big> | ||
| + | |||
| + | * Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death. | ||
| + | * | ||
Revision as of 19:08, 13 June 2017
This protocol is modified from section 3.1.2 of Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells and the first part of the following video with accompanying protocol DNA Electroporation, Isolation and Imaging of Myofibers | Protocol.
Materials
- Tweezers
- Standard operating scissors
- Surgical scissors
- Forceps
- Dissection stage with 7 pins
- Anesthetic
Flexor Digitorum Brevis Muscle Extraction
- Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.
