Difference between revisions of "Preparation of PEI Stocks"
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* Aliquot 500 uL to 1000 uL and store in -80C | * Aliquot 500 uL to 1000 uL and store in -80C | ||
* Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed | * Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed | ||
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| + | [[ Category: CRISPR ]] | ||
| + | [[ Category: Cell Culture ]] | ||
| + | [[ Category: Tissue Culture ]] | ||
| + | [[ Category: Transfection ]] | ||
| + | [[ Category: Molecular Biology ]] | ||
Revision as of 15:42, 8 December 2017
Materials
- Polyethylenimine 25kDa; from Sigma catalog number 764965-1G made at 1 mg/mL
Preparation
- Dissolve 100 mg in 100 mL sterile ddH2O
- Stir while slowly adding HCl to pH 7.0
- Mix for 10 minutes and then recheck pH
- Filter sterilize through 0.22 um filter
- Aliquot 500 uL to 1000 uL and store in -80C
- Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed
