Difference between revisions of "Phosphate Buffered Saline"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na<sub>2</sub>HPO<sub>4</sub>. * Add 0.24 g of KH<sub>2</sub>PO<sub>4...") |
Davebridges (Talk | contribs) (→Recipe) |
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==Recipe== | ==Recipe== | ||
| − | * Start with | + | * Start with 400ml of distilled water: |
| − | * Add | + | * Add 4 g of NaCl. |
| − | * Add 0. | + | * Add 0.72 g of KCl. |
| − | * Add 1. | + | * Add 1.72 g of Na<sub>2</sub>HPO<sub>4</sub>. |
| − | * Add 0. | + | * Add 0.12 g of KH<sub>2</sub>PO<sub>4</sub>. |
* Adjust the pH to 7.4 with HCl. | * Adjust the pH to 7.4 with HCl. | ||
| − | * Add distilled water to a total volume of | + | * Add distilled water to a total volume of 500 mL. |
Revision as of 21:54, 25 January 2018
Recipe
- Start with 400ml of distilled water:
- Add 4 g of NaCl.
- Add 0.72 g of KCl.
- Add 1.72 g of Na2HPO4.
- Add 0.12 g of KH2PO4.
- Adjust the pH to 7.4 with HCl.
- Add distilled water to a total volume of 500 mL.
