Difference between revisions of "Phosphate Buffered Saline"
From Bridges Lab Protocols
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* Add 4 g of NaCl. | * Add 4 g of NaCl. | ||
* Add 0.72 g of KCl. | * Add 0.72 g of KCl. | ||
| − | * Add | + | * Add 0.72 g of Na<sub>2</sub>HPO<sub>4</sub>. |
* Add 0.12 g of KH<sub>2</sub>PO<sub>4</sub>. | * Add 0.12 g of KH<sub>2</sub>PO<sub>4</sub>. | ||
* Adjust the pH to 7.4 with HCl. | * Adjust the pH to 7.4 with HCl. | ||
* Add distilled water to a total volume of 500 mL. | * Add distilled water to a total volume of 500 mL. | ||
Latest revision as of 21:55, 25 January 2018
Recipe
- Start with 400ml of distilled water:
- Add 4 g of NaCl.
- Add 0.72 g of KCl.
- Add 0.72 g of Na2HPO4.
- Add 0.12 g of KH2PO4.
- Adjust the pH to 7.4 with HCl.
- Add distilled water to a total volume of 500 mL.
