Difference between revisions of "Deparaffinizing Histology Slides"
From Bridges Lab Protocols
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#Xylene: 2 x 3 minutes | #Xylene: 2 x 3 minutes | ||
| − | #Xylene 1: | + | #Xylene 1:1 with 100% Ethanol: 3 minutes |
#100% Ethanol: 2 x 3 minutes | #100% Ethanol: 2 x 3 minutes | ||
#95% Ethanol: 3 minutes | #95% Ethanol: 3 minutes | ||
Revision as of 20:22, 16 July 2018
Materials
- Xylene
- 100% Ethanol
- 95% Ethanol
Method
- Use slide rack/holder
- Xylene: 2 x 3 minutes
- Xylene 1:1 with 100% Ethanol: 3 minutes
- 100% Ethanol: 2 x 3 minutes
- 95% Ethanol: 3 minutes
- 70% Ethanol: 3 minutes
- 50% Ethanol: 3 minutes
- Running cold tap water to rinse
Keep the slides in the tap water until ready to perform staining protocol.
