Difference between revisions of "Measuring Cross-Sectional Area (CSA)"
From Bridges Lab Protocols
| Line 5: | Line 5: | ||
#Use the Scrolling tool(hand) to grab and move the picture | #Use the Scrolling tool(hand) to grab and move the picture | ||
#Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end | #Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end | ||
| − | #Analyze | + | #"Analyze" |
| − | #Set scale | + | #"Set scale" |
| − | #Known distance: 200 (for 20x) | + | #"Known distance:" 200 (for 20x) |
| − | #Unit of length: um | + | #"Unit of length:" um |
| − | #OK | + | #"OK" |
#Adjust the zoom. Start in upper left hand corner | #Adjust the zoom. Start in upper left hand corner | ||
| − | #Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press M | + | #Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M" |
| − | #Continue tracing and press M after each fiber trace | + | #Continue tracing and press "M" after each fiber trace |
| − | #Results | + | #"Results" |
| − | #Summarize | + | #"Summarize" |
Latest revision as of 18:43, 27 August 2018
Using ImageJ
- Drag your image into ImageJ.
- Use the Magnifying glass. Click on the image to zoom in and right click to zoom out
- Zoom in on the scale on your image
- Use the Scrolling tool(hand) to grab and move the picture
- Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end
- "Analyze"
- "Set scale"
- "Known distance:" 200 (for 20x)
- "Unit of length:" um
- "OK"
- Adjust the zoom. Start in upper left hand corner
- Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M"
- Continue tracing and press "M" after each fiber trace
- "Results"
- "Summarize"
