Difference between revisions of "Preparation of Chloroquine Stocks"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Wrote instructions for making chloroquine) |
Davebridges (Talk | contribs) (updated due to uptick in COVID search results for chloroquine) |
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| + | Our lab uses chloroquine to block lysosomal acidification in cell lines only. '''This is not an appropriate preparation for human consumption'''. For information regarding chloroquine related to COVID-19 please see the [https://www.fda.gov/media/136535/download FDA Fact Sheet for Healthcare Providers] | ||
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==Materials== | ==Materials== | ||
* Chloroquine. Sigma catalog# C6628-25G | * Chloroquine. Sigma catalog# C6628-25G | ||
Latest revision as of 20:02, 7 April 2020
Our lab uses chloroquine to block lysosomal acidification in cell lines only. This is not an appropriate preparation for human consumption. For information regarding chloroquine related to COVID-19 please see the FDA Fact Sheet for Healthcare Providers
Materials
- Chloroquine. Sigma catalog# C6628-25G
Protocol
- Dissolve 0.129g in 10 mL of sterile ddH2O
- Filter sterilize through a 0.22 um filter
- Aliquot to 50 uL in tissue culture freezer
- Discard thawed aliquots after ~ 2 months.
