Difference between revisions of "PCR Analysis of Tail DNA"
From Bridges Lab Protocols
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==Protocol== | ==Protocol== | ||
| − | Use the following | + | Use the following Volumes per Reaction: |
| + | |||
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | ||
| + | |||
Forward Primer: .4ul | Forward Primer: .4ul | ||
| + | |||
Reverse Primer: .4ul | Reverse Primer: .4ul | ||
| − | dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | + | |
| + | dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | ||
| + | |||
Sterile water: 13.6 uL | Sterile water: 13.6 uL | ||
| + | |||
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | ||
| + | |||
Template: 1 uL | Template: 1 uL | ||
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | ||
Revision as of 16:28, 5 June 2009
see Genotyping Details for strain specific details
Materials
Protocol
Use the following Volumes per Reaction:
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)
Forward Primer: .4ul
Reverse Primer: .4ul
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)
Sterile water: 13.6 uL
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)
Template: 1 uL
see Preparing an Agarose Gel for details on preparing a DNA gel
