Difference between revisions of "Glucose Tolerance Test"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added glucose strip ordering information) |
Davebridges (Talk | contribs) (added link to fasting insulin) |
||
| Line 11: | Line 11: | ||
#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. Put water bottle on cage. | #Bring mice to procedure room. Put water bottle on cage. | ||
| − | #Weigh mice, mark tails as necessary and take fasting glucose measurement. | + | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capilary about 2/3 full with blood. |
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | ||
Revision as of 14:43, 16 June 2009
Materials (Bring Downstairs)
- Glucometer - AccuChek Advantage
- Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
- Timer
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room. Put water bottle on cage.
- Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for Measuring Fasting Insulin by filling a heparinized capilary about 2/3 full with blood.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
- Immobilize mouse and restrain tail with one hand
- Aim needle between the midline and the hip bone
- Insert syringe (do not inject) into cavity
- Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
- At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein
