Difference between revisions of "Finding and Ordering BAC Clones"

Revision as of 15:26, 27 July 2009

Find Clone with UCSD Genome Browswer

1. Go to [UCSC Genome Browser]
2. Click on Genome Browser
3. Select Mouse (or whatever species you need)under genome
4. Type gene name in the search box
5. Select appropriate item from search results
6. Adjust tracks as necessary, being sure to include BAC End Pairs under Mapping and Sequence Tracks
7. Zoom out as necessary to find ends of BAC clones
8. Print out and save BAC clone choices

Find Clone with BLAST

1. Go to blast website (http://www.ncbi.nlm.nih.gov/blast)
2. Select Mouse (or whichever species) under BLAST assembled genomes
3. Select clone end sequences under database
4. Select appropriate program (BLASTN if using a nucleotide query, TBLASTN if using a protein query)
5. Enter sequence or accession number in the search box

Ordering BAC Clones

1. Go to BACPAC website (http://bacpac.chori.org/order_clones.php)
2. Enter clone name and submit to verify
3. Can order clones through LSI purchasing (vendor is listed as BACPAC-chor)