Difference between revisions of "Finding and Ordering BAC Clones"
From Bridges Lab Protocols
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#Go to [[http://genome.ucsc.edu UCSC Genome Browser]] | #Go to [[http://genome.ucsc.edu UCSC Genome Browser]] | ||
#Click on Genome Browser | #Click on Genome Browser | ||
− | #Select Mouse (or whatever species you need)under genome | + | #Select Mouse (or whatever species you need) under genome |
#Type gene name in the search box | #Type gene name in the search box | ||
#Select appropriate item from search results | #Select appropriate item from search results |
Revision as of 15:28, 27 July 2009
Find Clone with UCSD Genome Browswer
- Go to [UCSC Genome Browser]
- Click on Genome Browser
- Select Mouse (or whatever species you need) under genome
- Type gene name in the search box
- Select appropriate item from search results
- Adjust tracks as necessary, being sure to include BAC End Pairs under Mapping and Sequence Tracks
- Zoom out as necessary to find ends of BAC clones
- Print out and save BAC clone choices
Ordering BAC Clones
- Go to BACPAC website (http://bacpac.chori.org/order_clones.php)
- Enter clone name and submit to verify
- Can order clones through LSI purchasing (vendor is listed as BACPAC-chor)