Difference between revisions of "Collecting and Storing Mouse Serum"
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Latest revision as of 15:54, 18 January 2024
Materials
- Heparinzed capilaries (Fisher cat# 22-362574)
Protocol
- Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
- Transfer blood to labelled eppendorf tubes.
- Let blood clot for 30 min on ice.
- Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
- Gentry remove liquid layer and store at -80
