Difference between revisions of "Acrylamide Solution"
From Bridges Lab Protocols
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[[Category:SDS-PAGE]] | [[Category:SDS-PAGE]] | ||
[[Category:Electrophoresis]] | [[Category:Electrophoresis]] | ||
| − | [[Category: | + | [[Category:Buffer]] |
For 1L: | For 1L: | ||
*181.66g Tris | *181.66g Tris | ||
Revision as of 18:55, 3 August 2009
For 1L:
- 181.66g Tris
- 40 mL 10% SDS (or 4 g SDS)
- Add water to near 1L
- Adjust pH to 8.8
- Bring volume up to 1L
