Difference between revisions of "FM4-64 Labeling of Yeast Cells"
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Revision as of 19:57, 5 October 2009
Materials
Protocol
- Grow and overnight culture of yeast in the appropriate media.
- Spin cells down and resuspend in FM4-64 for 1h.
- Quench cells for 3h.
- Mount cells on coverslip and analyze by fluorescent microscopy
