Difference between revisions of "KRBH Buffer"
From Bridges Lab Protocols
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Latest revision as of 18:27, 14 October 2009
| Final Concentration | per 500 mL | Stock | Location | |
|---|---|---|---|---|
| HEPES pH 7.4 | 30mM | 15mL | 1M | |
| Sodium bicarbonate | 10mM | 5mL | 1M | Make fresh from powder |
| Sodium chloride | 120mM | 15mL | 4M | |
| Potassium phosphate | 4mM | 2mL | 1M | |
| Magnesium sulfate | 1mM | 500uL | 1M | |
| Calcium chloride | 1mM | 500uL | 1M |
- Bring up to 500 mL with ddH20
- Sterile filter buffer and store at 4C
