Difference between revisions of "HNTG Buffer"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Created page with ' Category:Buffer Category:Immunoprecipitation {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...') |
Davebridges (Talk | contribs) |
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| NaCl || 150 mM || 375 uL || 4M || Shelf | | NaCl || 150 mM || 375 uL || 4M || Shelf | ||
|- | |- | ||
| − | | Glycerol || | + | | Glycerol || 10% || 1 mL || || Bench |
|- | |- | ||
| Triton X-100 || 1% || 0.5 mL || 20% || Shelf | | Triton X-100 || 1% || 0.5 mL || 20% || Shelf | ||
Latest revision as of 14:50, 4 November 2009
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| HEPES, pH 7.4 | 50 mM | 500 uL | 1M | 4C |
| NaCl | 150 mM | 375 uL | 4M | Shelf |
| Glycerol | 10% | 1 mL | Bench | |
| Triton X-100 | 1% | 0.5 mL | 20% | Shelf |
| Protease Inhibitor | 1 tablet |
