Difference between revisions of "Rab5 FRET Assay"
From Bridges Lab Protocols
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Latest revision as of 14:18, 16 September 2010
Materials
- mCitrine-Rab5BD (from EEA1/NT)
- mCFP-Rab5A
- Acid etched Coverslips (see Acid Etching Coverslips)
- Cells of interest
Protocol
- Plate out ~150 000 cells per well in a 6 well dish with an acid etched coverslip
- The next morning transfect cells with Fugene, Lipofectamine or other protocol
- That evening replace with fresh media (and serum starve if doing insulin/growth factor stimulation
