Difference between revisions of "MTM1 Genotyping"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added sample pcr result) |
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| + | [[ Category:Genotyping ]] | ||
| + | [[ Category:Mouse Work]] | ||
| + | [[ Category:PCR]] | ||
| + | [[ Category:DNA]] | ||
| + | [[ Category:Molecular Biology]] | ||
| + | |||
See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153 | See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153 | ||
Latest revision as of 15:17, 21 January 2013
Primers
- MTM1 Fwd: GGC CCC ATT AAG GGA AGA GT
- MTM1 WT Rev: GAT CTT CTG GGA AAG GCA CA
- MTM1 Mut Rev: GCC AGA GGC CAC TTG TGT AG
PCR Program
- 94 for 2 min
- 10 cycles of:
- 94 for 20sec
- 65 for 15sec with -0.5 C per cycle
- 68 for 10sec
- 28 cycles of:
- 94 for 15sec
- 60 for 15sec
- 72 for 10sec
- 72 for 1 min
- 4 hold
