Transfer Buffer: Difference between revisions

From Bridges Lab Protocols
Jump to navigation Jump to search
copied recipe from saltiel lab buffers
(No difference)

Revision as of 14:57, 12 April 2013

10X Transfer Buffer

To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:

  • 100 mL 10X Buffer
  • 200 mL Methanol
  • 700 mL Water
Component Weight per 10 L Catalog Number
Tris Base 303 g Sigma T6066-5KG
Glycine 1441 g Sigma G7126-5KG